How to Use a CE Standard Liquid Nitrogen Storage Tank for Optimal Cryopreservation?
Step 1: Prepare the Liquid Nitrogen Storage Tank.
Make sure the CE standard liquid nitrogen storage tank is clean and free of debris before use. Check the levels of liquid nitrogen and ensure that the tank is properly vented to avoid pressure build-up.
Step 2: Prepare the Cryovials.
Label each cryovial with identification information before filling. Use disposable pipettes to transfer the cells or tissue into the cryovials. Leave a small amount of headspace in the cryovial to avoid pressure build-up during freezing.
Step 3: Load Cryovials into the Storage Tank.
Carefully place the cryovials into the liquid nitrogen storage tank. Be sure to use the proper protective gear, including gloves and eye protection, when handling cryovials.
Step 4: Monitor and Maintain the Storage Temperature.
Monitor the storage temperature regularly. Ensure that the temperature remains at -196°C by regularly adding liquid nitrogen to maintain levels. Avoid opening the storage tank unnecessarily.
Step 5: Retrieving the Sample.
When retrieving samples, wear protective gear and use appropriate handling tools. Thaw the sample quickly and carefully in a water bath set to the recommended temperature. Do not refreeze the sample.
Using a CE standard liquid nitrogen storage tank is crucial for ensuring optimal cryopreservation. Following these steps will help ensure that your samples remain viable and intact. Proper maintenance and monitoring of the storage tank will also help avoid any unnecessary contamination or loss of samples.
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