What is the difference between T25 and T75 flasks?

 

The Difference Between T25 and T75 Flasks

T25 and T75 flasks are both commonly used in cell culture, but they differ in size and volume capacity.

T25 Flask:

A T25 flask is smaller in size compared to a T75 flask. It has a working volume of about 25 ml and a surface area of approximately 25 cm^2. T25 flasks are typically used for small-scale experiments or when working with limited cell numbers. They are suitable for experiments that require less cell growth or when using cell lines that grow slowly.

T75 Flask:

On the other hand, a T75 flask is larger in size with a working volume of around 75 ml and a surface area of about 75 cm^2. T75 flasks are commonly used for routine cell culture work, such as cell expansion, propagation, and maintenance. They provide a larger surface area for cell growth, allowing for increased cell proliferation and higher cell yields compared to T25 flasks.In summary, the main difference between T25 and T75 flasks lies in their size and volume capacity. T25 flasks are smaller and used for small-scale experiments or with limited cell numbers, while T75 flasks are larger and more commonly used for routine cell culture work requiring higher cell yields. It is important to select the appropriate flask size based on the specific requirements of your experiment or cell culture protocol.

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